What is the difference between GAL-S, GAL-L and GAL1 promoters in the pFunk expression vector series?
Modified on: Thu, 4 Oct, 2012 at 7:21 AM
The GAL-S and GAL-L promoters are variants of the commonly used inducible GAL1 promoter. Like GAL1p they are repressed by glucose and induced by galactose. The strength of expression is GAL-S < GAL-L < GAL1. Note that when using galactose to induce expression from these promoters it is recommended to use a galactose/raffinose mix to ensure robust growth of yeast.
Please download the publications below to learn more about the pFunk vectors.
Was this answer helpful?
Sorry we couldn't be helpful. Help us improve this article with your feedback.